Acute Oral Toxicity Assessment of Diarrhea Stop Herbal Product in Rats: A Single-Dose Limit Test Study

Abstract

Introduction: Diarrhea is a common gastrointestinal disorder that significantly impacts health and quality of life worldwide. Herbal medicine offers a promising approach to managing diarrhea, combining traditional knowledge with modern pharmacological evidence.
Objective: This study aims to assess the acute toxicity by calculating the LD50 of Diarrhea Stop in rats.
Materials and Methods: Seven groups containing six rats (200 - 250 g) were used in this study. All animals were treated orally once and different doses (control, 20, 88.6, 177, 354, 1063, 5000 mg/kg) were administered. Animals were weighed before the dose administration. All the animals were kept under continuous observation for 6 hours after the administration of the dose, for any change in behavior or physical activity. After 24 hrs, all rats were sacrificed and autopsied.
Results: The results reveal that the diarrhea stop is not toxic even at 5,000 mg/kg or 20 mg/kg, that is, 35 times higher than the human dose in experimental animals. The animals received 5.000 mg/kg orally, which was not found to cause any mortality, and no changes were observed in wellness parameters used for evaluation of toxicity. However, the low doses did not produce any pronounced effect. Autopsy revealed that no changes were observed in organ structure and weight.
Conclusion: The present study indicates that the LD50 of Diarrhea Stop is more than 5000mg/kg in rats, as this dose did not cause mortality, no changes were observed in wellness parameters used for evaluation of toxicity, and no change in organ structure.